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JAMB > Volume 1, Number 1, June 2017

Proteomic Responses of the Cyanobacterium Nostoc Muscorum under Salt and Osmotic Stresses

Download PDF  (486.2 KB)PP. 1-22,  Pub. Date:June 13, 2017
DOI: 10.22606/jamb.2017.11001

Author(s)
D. Gupta, K. Bhardwaj, R. Gothalwal, S. Bhargava
Affiliation(s)
Department of Biotechnology and Bioinformatics Centre, Barkatullah University, Bhopal 462026 M.P.; Division of Microbiology, Department of Botany, Government Motilal Science College, Bhopal 462008 M.P; Department of Biotechnology and Bioinformatics Centre, Barkatullah University, Bhopal 462026 M.P; Division of Microbiology, Department of Botany, Government Motilal Science College, Bhopal 462008 M.P
Abstract
In this paper, we examined the effect of salt stress (NaCl) and osmotic stress (sucrose) on proteomic level in the diazotrophic cyanobacterium Nostoc muscorum. The aim of this study is to compare proteins appeared in control vs. salt treated, control vs. sucrose treated and salt treated vs. sucrose treated cultures. In the salt treated cultures about 37 proteins were expressed differentially out of these only 5 proteins have shown fold regulation of 1.5 or more. About 141 proteins were found to express independently in control and about 554 proteins were express independently in salt treated culture. When we compared proteins in control and sucrose treated cells, it was reported that about 37 protein spots were express differentially, out of these only 7 proteins have fold regulation 1.5 or more. The independently expressed proteins appeared on gel are 141 and 186 respectively. Similarly, when we compared proteins appeared in salt and sucrose treated cells, it was reported that about 54 proteins were express differentially, out of these 10 proteins have fold regulation 1.5 or more. About 537 protein spots were independently present in salt treated cells and about 186 proteins were independently present in sucrose treated cells. In addition, the differentially expressed proteins and their identification with their functional group have also been discussed.
Keywords
Nostoc muscorum, osmotic stress, proteomic, salt stress
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