Journal of Advances in Nanomaterials

Download PDF (1227 KB) PP. 95 - 104 Pub. Date: December 20, 2016

DOI: 10.22606/jan.2016.12006

• Min Yang¹, Junjie Sun¹, Yanzhong Zhao^{1, 2,**
  1}The Third Xiangya Hospital, Central South University, Changsha 410013, China
• Zewen Song¹, Haibin Zhang¹, Shaihong Zhu<sup>1, 2
  
  2</sup>Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China

This study was to investigate the biological safety of arginine-modified hydroxyapatite nanoparticles (HAP/Arg) as a gene carrier for gene therapy. Experiments were conducted on normal human vascular endothelial cells (HAEC) and human tumor cells (Hela cells) to study the biological safety of HAP/Arg at subcellular level, cellular level, and overall level of animal. Cell proliferation, structural integrity of cell membrane, acute toxicity of HAP/Arg and animal reproductive toxicity of HAP/Arg were all examined. The results showed that HAP/Arg of various doses did not significantly affect normal growth and cell membrane structure of HAEC cells and Hela cells, when these cells were treated for different duration. Acute toxicity experiments and general reproductive toxicity experiments on animals also revealed no significant difference between each HAP/Arg dose group and control group. These results could lay the foundation for the construction of a novel and safe HAP gene delivery system.

Hydroxyapatite, gene carrier, arginine-modification, biological safety.

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