Isaac Scientific Publishing

Modern Clinical Medicine Research

Determination of the Dose of Erythrosine B as a Preparation for Inducing the Experimental Rats to Be Spastic

Download PDF (168.7 KB) PP. 9 - 12 Pub. Date: July 20, 2019

DOI: 10.22606/mcmr.2019.32001

Author(s)

  • Marina Indriasari*
    Faculty of Human Ecology, Bogor Agricultural University, Bogor, West Java, Indonesia
  • Hardinsyah
    Faculty of Human Ecology, Bogor Agricultural University, Bogor, West Java, Indonesia
  • Lilik Kustiyah
    1Faculty of Human Ecology, Bogor Agricultural University, Bogor, West Java, Indonesia
  • Bambang Pontjo Priosoeryanto
    Faculty of Veterinary, Bogor Agricultural University, Bogor, West Java, Indonesia
  • Ferial Hadipoetro Idris
    Faculty of Medicine and Health University of Muhammadiyah, Jakarta, Indonesia

Abstract

This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.

Keywords

Dose, Erythrosine B, spastic.

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